1/17/2024 0 Comments Western blot test for hiv ppt![]() Particle agglutination, dot/blot Western blot Antigen & Ab/Ag Incidence assays Direct Virus Detection Available Assays: There is a large range of assays available, especially for anti-HIV screening and HIV-diagnosis. plasma RNA (viral load) resolution of inconclusive serology / neonatal subtyping drug resistance monitoringħ Available Assays EIAs including Direct Virus Detection rapid, simple HIV antigen– serology - In isolation - Diagnosis of primary infection viraemia Virus culture / isolation Nucleic acid detection - (NAT) Clinical uses Proviral DNA vs. + DNA (RNA) DIAGNOSIS MANAGEMENTĦ HIV Testing - Direct Detection of Virus Other tests monitor the status of immune function indirectly but these are used widely in HIV management. ![]() Viral load estimations are used to gauge prognosis and to monitor therapy. The emphasis in using anti-HIV tests has been somewhat eclipsed by the news around tests that quantify viral load. There has been a change in test usage with the introduction of molecular-based tests. The detection of HIV infection by antibody tests is an indirect confirmation of infection. To identify positively the presence of anti-HIV, two assays using different technology and different antigen preparations, should be used. Virus Detection EIA Simple, rapid tests Immunoblots Incident assays Antibody Antigen Detection HIV Assays - Methodologies and Utilisation: The diagnosis of HIV infection usually rests on the identification of specific antibody to HIV in plasma or serum. Symptoms symptoms HIV proviral DNA HIV antibodies ‘window’ period HIV viral load HIV-1 p24 antigen / 1° infection weeks years Time following infectionĥ HIV Assays: Methodologies FOR THE DIAGNOSIS (DETECTION) They should be used in combination (strategies) Combinations must be consistentģ Laboratory Tests diagnosis of infection prognostic markersĪcute, recent, established or late stage disease prognostic markers monitoring of ARV therapies immunological and virological markers toxicities diagnosis of opportunistic infections drug resistance testing 1 HIV Testing CDC power point edited by M.
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